Здоровый

Здоровый

Terminal Sialic Acids are an Important Determinant of Pulmonary Endothelial Barrier Integrity

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In some experiments the bacterial surface was desialylated with 13· In the earlier set of experiments that utilized neuraminidase, we noted that, following neuraminidase treatment, there were typically fewer cells in the dish, indicating that cells had lost cell-cell and/or cell-matrix adhesions. Sialic acid quantitation was carried out using the Sialic Acid (NANA) Assay kit … Read more

For Testing Bioactivity of PolySia AvDP20

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For experiments, PC-12 cells were collected via 0.25% trypsin (Gibco) and seeded for culturing in collagen IV (Sigma-Aldrich) coated 4-chamber slides (Nunc Lab-Tek, Merck). PC-12 cells grew as small clusters in suspension and were maintained in a density 5 × 105 cells/ml in culture flasks coated by 0.1% collagen type IV (Sigma-Aldrich). For testing bioactivity … Read more

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